Fig. 5.

LMP1 and LMP1 ID induce nuclear p52 and RelB accumulation; LMP1 AA is less active. HEK293T were transfected with expression vectors for WT and mutant LMP1 as in Fig. 4A. Nuclear and cytoplasmic fractions were analyzed by SDS/PAGE and immunoblot for p52, RelB (Santa Cruz Biotechnology), and tubulin as a control for fractionation and cytoplasmic protein levels. Total cell lysates (Lower) were also immunoblotted for RelB. Asterisks indicate nonspecific bands. The results shown in these blots were consistent with those found in similar repeat experiments.