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. Author manuscript; available in PMC: 2011 Jul 22.
Published in final edited form as: Hepatology. 2010 Jul;52(1):47–59. doi: 10.1002/hep.23671

Fig. 1. HCV increases the level of ROS.

Fig. 1

Huh7 cells transfected with virus-producing JFH1 HCV RNA or mock-transfected were analyzed for H2O2 by p-hydroxyphenylaminoacetic acid dimerization assay (A) and intracellular superoxide by measuring 2-OH-E+ by HPLC (B). Menadione (50 μM) plus diethyldithiocarbamate (3 mM, inhibitor of superoxide dismutase; added 30 min prior to HE) served as the positive control for the detection of 2-OH-E+ (B). When used, DPI was added at 10 μM concentration 30 min prior to the assays. Catalase was added at the final concentration of 200 U/ml. Data were normalized by total protein. *, a indicate statistically significant difference from HCV− or HCV+ controls, respectively (P < 0.05); N.D. indicates “non-detectable” (below baseline).