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. Author manuscript; available in PMC: 2011 Jul 22.
Published in final edited form as: Hepatology. 2010 Jul;52(1):47–59. doi: 10.1002/hep.23671

Fig. 3. Hepatocyte Nox1 and Nox4 proteins are increased with HCV.

Fig. 3

Huh7 cells were transfected with virus-producing full-length JFH1 RNA or mock-transfected, or infected with medium from the HCV RNA and GND RNA-transfected cells, and analyzed for Nox4 (A) and Nox1 (B) protein levels by western blots. Human liver samples from HCV-infected and non-infected donors (National Disease Research Exchange, NDRI) were also analyzed for Nox1 and Nox4 proteins by western blots (C). β-actin was analyzed as loading control. (D) Nox1 (50 kDa) and Nox4 (65 kDa) protein levels in control and JFH1 cells (left panel), and Nox1 and Nox4 proteins (> 60 kDa) in human liver tissues (right panel) were quantified by densitometry and normalized by the level of β-actin. * indicates statistically significant difference from the controls (P < 0.05). Arrows indicate expected sizes of Nox1 and Nox4.