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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Curr Protoc Mol Biol. 2011 Jul;CHAPTER:Unit23.14. doi: 10.1002/0471142727.mb2314s95

Figure 2. Flow of BAC targeting with targeting cassette.

Figure 2

On the targeting cassette the left arm of homology (LAH) and right arm of homology (RAH) add a homologous region to the targeting cassette that matched with the left and right flanking portion of the targeted site on the BAC. The genotyping primers (GT) must be located outside of the arms of homology. If the selection marker is flanked by FRT sites then use EL250 to remove selection marker, flanked by LoxP sites use EL350.