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Scheme for generating constitutive mutants in human embryonic kidney (HEK) cells and EMSA of parental and eight mutant clones. Cell extracts were made from parental C6 and mutant cells. Extracts were analyzed by EMSA for the ability of NF-κB to bind to the NF-κB-consensus sequence of the IP-10 gene. The faster moving band is the p50/p65 heterodimer, and the slower moving band is the p65 homodimer.
