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Dominance and complementation. Each of the constitutive mutants tested was cotransfected independently with pBABE-Hygro and pBABE-Puro. The mutants P2Z1, Z2, and Z5 were fused either to parental C6 or C8 cells (dominance test) or to each other by using polyethylene glycol. The heterokaryons were selected in the presence of both hygromycin and puromycin. Pools of the clones resulting from each fusion were analyzed by EMSA by using an IP-10 probe.
