Fig. 6.

Phenotype of complemented clones of RasC6 cells. (A) EMSA for NF-κB in RasC6 cells and enolase 1-complemented clones (P2C8, P3C1, and P4C6; no cDNA was recovered from P1C4), untreated or treated with IL-1 for 20 min. (B) Enolase 1 inhibits IL-1-stimulated NF-κB promoter activity in the RasC6 cell line. Cells were cotransfected transiently with increasing amounts of pCR 3.1 enolase 1 and with 1 μg of the NF-κB-dependent reporter E-selectinluciferase and pSV2-β-gal plasmid. Forty-eight hours posttransfection, cells were incubated with IL-1 for 4 h. Luciferase activity was normalized to β-galactosidase to correct for transfection efficiency.