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| Mutagenized pool | No. of constitutive mutants | Mutants selected for further analysis |
|---|---|---|
| C6P1 | 12 | C6P1Z1 and Z12 |
| C6P2 | 2 | C6P2Z1 and Z2 |
| C8P1 | 5 | Z3 and Z23 |
| C8P4 | 5 | Z5 |
| C18P3 | 1 | Z13 |
C6, C8, and C18 are three different initial clones. P1—P4 are independently mutagenized pools. Z1—Z23 are individual zeocin-resistant clones.
