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. 2011 Jun 8;6:13. doi: 10.1186/1747-1028-6-13

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Copyright ©2011 Islam et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Gcn5 promoter occupancy is kept at an equilibrium in WT cells, but increases over time in hda1 cells. (A) Western analyses of Gcn5-HA expression in gcn5Δ and gcn5Δ hda1Δ cells following a 5 hour 4% galactose-induction. Antibodies against GAPDH were used as a load control. (B) A galactose-induction time-course was performed in gcn5Δ and gcn5Δ hda1Δ cells expressing GAL_pro-GCN5-HA. Protein samples were removed at the times shown for Western analyses with antibodies against HA and GAPDH. (C) From the time-course described above, samples were also removed for ChIP. Recovered DNA was used as a template in "end point" PCR reactions. S, sample with antibody; C, control without antibody; I, 10% lysate input. (D) The gel in (C) was scanned, analyzed using ImageJ and plotted.