Figure 3.

H₂O₂ causes insolubilisation of the grapevine extensin, GvP1. A) Superose-12 chromatography of EIBMPs (saline eluates of 35 mg (FW) cells) from untreated cells (trace control), cells incubated with 100 mM H₂O₂ (trace + H₂O₂). A chromatogram of pure GvP1 is also indicated as a reference. A time course assay of GvP1 insolubilisation in muro is depicted in the inset. B) SP-Sepharose chromatography of whole EIBMPs. Fractions enriched in GvP1 and subject to TCA fractionation for the purification of GvP1 are delimited by solid grey lines.