Table 1.
H2O2-mediated changes in the amino acid composition of saline-insoluble protein in the cell wall matrix
| a.a. | GvP1 | Control walls | H2O2-treated walls | H2O2-induced changes in walls |
|---|---|---|---|---|
| (mol %) | (nmole.mg-1) | (nmole.mg-1) | (nmole.mg-1) | |
| Hyp | 45.3 | 3.0 | 32.6 | 29.6 |
| Lys | 12.0 | 6.1 | 17.7 | 11.6 |
| Tyr | 8.4 | 9.5 | 14.9 | 5.3 |
| Ser | 8.0 | 37.5 | 43.5 | 6.0 |
| Pro | 5.7 | 30.8 | 36.0 | 5.2 |
The amino acid (a.a.) composition of pure grapevine extensin, GvP1 (mol %), is shown for comparison. The content of each amino acid in wall preparations was measured after saline extraction to remove saline-soluble EIBMPs and is given in nmole.mg-1 (DW) cell wall. The less abundant amino-acids of GvP1 are omitted for clarity.