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Extractability change of ADAM-1 during sperm maturation. Total SDS sperm protein extracts (A) or two different gel loading of purified biotinylated sperm surface proteins (B and C) from the proximal caput (Z2), distal caput (Z4), corpus (Z6) and distal cauda (Z9) were probed with the anti ADAM-1 C-terminal polyclonal antibody. Purified biotinylated surface proteins (D) were probed with the anti-ACE as controls for extraction and purification.
