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. 2011 Jun 20;8:48. doi: 10.1186/1742-4690-8-48

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Copyright ©2011 Melchers et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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SOSIP.R6-IZ-CD40L induces DC maturation. A. Monocyte-derived iDC were cultured for 48h in the presence of SOSIP.R6-IZ, SOSIP.R6-IZ-CD40L or control stimuli. The expression of the maturation marker CD83 was monitored by FACS. B. CD83 was measured on DC stimulated for 48 h with a combination of TNF-α/IL-1β and SOSIP.R6-IZ, SOSIP.R6-IZ-CD40L or control stimuli. Gray lines represent the isotype-matched control. Cells were double-stained for CD11c and CD83 and CD83 Histograms of cells gated on CD11c are shown (> 25,000 events).