Figure 1.

All Dacts run at higher than expected molecular weight and are phosphorylated in vivo. Lysates from HEK293 cells transiently transfected with plasmids expressing N-terminal FLAG-tagged murine Dact proteins, enzymatically treated as indicated, separated by SDS-PAGE, and visualized by immunoblotting with anti-FLAG antibody. All panels: lanes 1 & 3 untreated, lane 2 treated with phosphatase, Lane 4 treated with glycosylase. A, Dact1. B, Dact2. C, Dact3. In this and all subsequent figures, the number of replicate experiments is indicated in grey beneath each corresponding panel.