Fig. 3.

Frequent locations of original bioluminescent foci derived from single HSC. Single luc⁺ or luc⁺ GFP⁺ KTLS HSC were transplanted into lethally irradiated nontransgenic recipients along with 3 × 10⁵ nontransgenic Sca-1^- BM cells. BLI was performed on day 12–23 after transplantation, and at these time points, single bioluminescent foci were visualized from 23 of a total of 113 recipients at different time points; four recipients showed no single bioluminescent foci on days 12–23 but did show detectable levels of hematopoietic engraftment after 4 weeks and therefore were categorized as undetected at the early time point. (A) Frequent sites among different BM compartments where bioluminescent foci were initially detected. (B) The frequency of bioluminescent foci detected from spleen or BM compartments.