Table 1.

Assignment of the ¹H NMR spectral signals of the apolar fraction.

Peak number	Chemical shift (ppm)	Assignmenta
1	0.68	Total cholesterol C18H3
2	0.85/0.86	Total cholesterol C26H3/C27H3
3	0.86-0.91	Acyl groups CH3 (all except n-3)
4	0.92	Total cholesterol C21H3
5	0.98	n-3 acyl groups CH3
6	1.01	Free cholesterol C19H3
7	1.02	Esterified cholesterol C19H3
8	1.06-1.19	Multiple cholesterol protons
9	1.20-1.38	Acyl groups -(CH2)n-
10	1.50-1.65	Acyl groups -OCO-CH2-CH2- and H2O
11	1.79-1.88	Multiple cholesterol protons
12	1.97-2.10	Acyl groups -CH2-CH=CH-
13	2.24-2.38	Acyl groups -OCO-CH2-
14	2.72-2.88	Acyl groups =HC-CH2-CH=
15	3.28-3.38	Choline N(CH3)3
16	3.90-4.02	Glycerophospholipids N-CH2
17	4.10-4.32	Glycerol backbone -CH2OCOR
18	4.33-4.43	Phosphatidylcholine PO-CH2
19	4.50-4.66	Esterified cholesterol C3H
20	5.17-5.24	Glycerophospholipid backbone >CHOCOR
21	5.24-5.28	Glycerol backbone >CHOCOR
22	5.29-5.43	Acyl groups -CH=CH-

Numbers correspond to those in Figure 1.

^aProtons assignment based on [81]