Figure 5.

Bland-Altman plot showing level of agreement between technical replicates for natural log transformed RPKM D. simulans biological replicate 3. On the Y axis is the difference between technical replicates and on the X axis is the average between technical replicates. Green lines are the average of all differences +/- 1.96 (standard deviation of the differences). The red line is drawn at zero. The blue line is a loess fit. The discrepancy between technical replicates is a function of the estimated expression level. The horizontal line is drawn at an average coverage per nucleotide of 5. Bland-Altman plots for all the remaining comparisons among technical replicates are in Additional file 11.