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. 2011 Jun 13;12:310. doi: 10.1186/1471-2164-12-310

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Copyright ©2011 Tiago et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Real-time PCR analysis of genes detected in vanadate-treated VSa13 cells during mineralization (M vs MV) and proliferation (P vs PV) selected for validation. Genes were selected according to the following criteria: 3 up-regulated genes with 1.5-8 FC and 3 down-regulated genes with 1.5-8 FC. All changes in gene expression evaluated by qPCR were significant according to Student's test (p < 0.05). Pearson correlation coefficients, calculated comparing probes 1 and 2 from oligo-array, and comparing probes 1 and 2 from oligo-array with qPCR, are indicated by r. Genes selected for analysis were: ependymin-related protein (ERP), arginine N-methyltransferase (RNM), cyclin-dependent kinase (CDK), signal peptide CUB and EGF-like protein (SCUBE-like), tenascin (TNS) and thrombospondin (TBSD).