Table 2.

Gene description (according to SAPD database [28]), GO classification, FC and scores of common genes regulated during mineralization of VSa13 cells (FC_M) versus mineralization with vanadate (FC_MV)

Gene description	GOBP/MF	FCM	FCMV	Score
Photoreceptor outer segment all-trans retinol dehydrogenase [Source:IPIAcc:IPI00024598]	metabolic process/oxidoreductase activity	34,8	0,14	240,1
AMBP protein precursor [Source:Uniprot/SWISSPROTAcc:P02760]	-/-	47,4	0,36	131,5
Ependymin related protein-1 precursor [Source:IPIAcc:IPI00554718]	cell-matrix adhesion/calcium ion binding	15,2	0,14	111,8
Xaa-Pro aminopeptidase 2 precursor [Source:Uniprot/SWISSPROTAcc:O43895]	proteolysis, creatine metabolic process/metalloexopeptidase activity, hydrolase activity, creatinase activity	27,2	0,25	110,2
No match	-/-	28,2	0,31	89,73
Microtubule-associated proteins 1A/1B light chain 3C precursor [Source:Uniprot/SWISSPROTAcc:Q9BXW4]	-/-	12,6	0,14	89,63
Cell death activator CIDE-3 [Source:Uniprot/SWISSPROTAcc:Q96AQ7]	apoptosis/protein binding	7,77	0,17	45,71
No match	-/-	25,5	0,61	41,50
No match	-/-	12,7	0,32	39,97
PREDICTED: hypothetical protein [Danio rerio]	pore complex biogenesis/channel activity	18,3	0,52	35,20
Hypothetical protein LOC447879 [Source:RefSeq_peptideAcc:NP_001004618]	integrin-mediated signaling pathway/-	9,55	0,29	32,64
No match	-/-	17,5	0,54	32,31
Granulocyte-macrophage colony-stimulating factor receptor alpha chain precursor [Source:Uniprot/SWISSPROTAcc:P15509]	-/-	8,69	0,29	29,83
No match	-/-	5,77	0,21	27,18
IgGFc-binding protein precursor [Source:Uniprot/SWISSPROTAcc:Q9Y6R7]	cell adhesion/-	4,35	0,19	23,12
No match	-/-	6,28	0,28	22,13
Hydroxyacid oxidase 2 [Source:Uniprot/SWISSPROTAcc:Q9NYQ3]	metabolic process, electron transport/oxidoreductase activity	5,21	0,24	21,38
No match	-/-	7,70	0,39	19,85
No match	-/-	7,35	0,37	19,79
Phosphatase and actin regulator 4 isoform 1 [Source:RefSeq_peptideAcc:NP_001041648]	-/-	4,95	0,27	18,08
Ependymin related protein-1 precursor [Source:IPIAcc:IPI00554718]	cell-matrix adhesion/calcium ion binding	2,54	0,14	18,02
No match	-/-	8,95	0,52	17,26
No match	-/-	10,6	0,62	17,00
No match	-/-	6,53	0,39	16,57
Glucose-6-phosphate 1-dehydrogenase [Source:Uniprot/SWISSPROTAcc:P11413]	glucose metabolic process/glucose-6-phosphate dehydrogenase activity	4,30	0,27	16,05
Glutamate--cysteine ligase catalytic subunit [Source:Uniprot/SWISSPROTAcc:P48506]	-/-	4,71	0,29	16,04
Complement factor I precursor [Source:Uniprot/SWISSPROTAcc:P05156]	proteolysis/catalytic activity, serine-type endopeptidase activity, hydrolase activity, scavenger receptor activity	2,58	0,16	15,91
No match	-/-	5,66	0,41	13,71
No match	-/-	4,30	0,33	12,95
No match	-/-	3,64	0,29	12,77
No match	-/-	4,93	0,39	12,73
No match	-/-	5,41	0,43	12,69
Hypothetical protein LOC406276 [Source:RefSeq_peptideAcc:NP_998168]	-/calcium ion binding	5,07	0,40	12,54
Hypothetical protein LOC336637 [Source:RefSeq_peptideAcc:NP_956317]	cell redox homeostasis/-	4,66	0,38	12,17
Actin filament-associated protein 1-like 2. [Source:Uniprot/SWISSPROTAcc:Q8N4X5]	-/-	5,69	0,48	11,79
Transcribed locus, weakly similar to NP_175293.1 [Source:UniGeneAcc:Tru.931]	metabolic process/methyltransferase activity	2,82	0,24	11,69
No match	-/-	5,96	0,51	11,66
Ornithine carbamoyltransferase, mitochondrial precursor [Source:Uniprot/SWISSPROTAcc:P00480]	-/ornithine carbamoyltransferase activity, amino acid binding	4,17	0,37	11,38
No match	-/-	4,83	0,42	11,38
CDNA FLJ31025 fis, clone HLUNG2000501. [Source:Uniprot/SPTREMBLAcc:Q96ND9]	-/-	3,80	0,34	11,25
No match	-/-	3,71	0,34	11,05
No match	-/-	4,39	0,40	10,89
No match	-/-	4,76	0,45	10,59
Stromal cell-derived factor 1 precursor [Source:Uniprot/SWISSPROTAcc:P48061]	-/-	3,94	0,38	10,36
ADM precursor [Source:Uniprot/SWISSPROTAcc:P35318]	-/-	3,68	0,36	10,10
Beta-2-glycoprotein 1 precursor [Source:Uniprot/SWISSPROTAcc:P02749]	-/-	4,44	0,44	10,05
No match	-/-	3,37	0,34	10,03
No match	-/-	0,05	5,03	102,8
Signal peptide, CUB and EGF-like domain-containing protein 2 precursor [Source:Uniprot/SWISSPROTAcc:Q9NQ36]	-/calcium ion binding	0,09	7,02	78,01
No match	-/-	0,18	10,7	58,98
Actin, alpha skeletal muscle 1. [Source:Uniprot/SWISSPROTAcc:P68140]	-/structural molecule activity, ATP binding, protein binding	0,44	17,1	38,39
No match	-/-	0,05	1,77	36,79
No match	-/-	0,09	3,03	34,37
No match	-/-	0,45	14,9	33,47
Inter-alpha globulIn InhIbItor H3 [Source:IPIAcc:IPI00028413]	hyaluronan metabolic process/serine-type endopeptidase inhibitor activity	0,18	4,41	24,67
No match	-/-	0,09	1,92	21,62
Hypothetical protein LOC553753 [Source:RefSeq_peptideAcc:NP_001018560]	-/protein binding	0,13	2,84	21,14
No match	-/-	0,15	3,18	20,60
Tenascin precursor [Source:Uniprot/SWISSPROTAcc:P24821]	signal transduction/receptor binding	0,19	3,87	20,56
No match	-/-	0,14	2,77	20,33
Prolargin precursor [Source:Uniprot/SWISSPROTAcc:P51888]	-/-	0,16	3,06	18,89
Eukaryotic translation initiation factor 4E-1A-binding protein [Source:Uniprot/SWISSPROTAcc:Q98TT6]	negative regulation of translational initiation/eukaryotic initiation factor 4E binding	0,24	2,84	12,02
No match	-/-	0,40	4,76	11,87
No match	-/-	0,18	2,01	11,27
WNT1-inducible-signaling pathway protein 1 precursor [Source:Uniprot/SWISSPROTAcc:O95388]	regulation of cell growth/insulin-like growth factor binding	0,26	2,78	10,77
No match	-/	0,29	3,00	10,34

Genes were selected according to their patterns of expression (1^st group: FC_M > 1.5 and FC_MV < 0.67; 2^nd group: FC_M < 0.67 and FC_MV > 1.5) and highest scores (1^st group: FC_M/FC_MV > 10; 2^nd group: FC_MV/FC_M > 10). GO classification was subdivided in biological processes (BP) and molecular function (MF). Two-class SAM test were performed with FDR and FC limits lower than 5% and higher than 1.5.