Fig. 1.
Screening and validation of antibodies obtained from H-2Kb-tsA58 transgenic mouse-derived immortal splenocytes. Cells were released and resuspended in hybridoma medium (see Materials and Methods for details). ELISA against filamentous phage (fd-tet) and against recombinant phage capsid pIII protein was performed as described (2). BSA, hybridoma medium alone, preimmune serum, and secondary antibody served as negative controls. Immune polyclonal serum and anti-phage antibody served as positive controls. Antibodies were plated directly from culture supernatants and tested as follows: 1, preimmune serum at 1:400 dilution; 2, postimmune serum at 1:3,200 dilution; 3–10, supernatants derived different 6-, 24-, and 96-well plates, 2 weeks after plating of the spleen; 11, cultured medium alone as a negative control. Bars correspond to the mean. Standard errors of the mean were <1% of the mean.