Fig. 4.

Reactivity of supernatants from H-2K^b-tsA58 transgenic mouse-derived immortal splenocytes producing antibodies against phage proteins. Reactivity was evaluated after incubation with preimmune serum, postimmune serum, an anti-phage antibody, or supernatants containing anti-phage IgGs secreted from immortal splenocyte clones, as indicated. Cell culture medium alone served as an additional negative control. Antibodies reacting specifically against pIII and pVIII phage capsid proteins (arrows) were detected in supernatants from H-2K^b-tsA58 transgenic mouse-derived immortal splenocytes.