Figure 5.

Antioxidants increase proliferation of mMSCs. (A) Comparison of spontaneous formation of micronucleus in primary mMSCs supplemented with or without antioxidants (800 μM PBN+5 mM NAC). (B) Effects of antioxidants on number of mMSCs. Bone marrow cells were plated at a density of 2 × 10⁵ cells/cm² on 6-well and cultured under 2% O₂ with or without addition of antioxidants for 5 days, and cells were counted by hemocytometer. (C) Effects of H₂O₂ on the proliferation of mMSCs. Primary mMSCs were replated at 1000 cells/cm² and cultured for two days, then H₂O₂ were added and cell number counted after 24 h. (D) Micronucleus analysis on mMSCs. (E) Karyotype analysis of mMSCs on plastic or ECM treated with H₂O₂. Data are expressed as the mean ± SD. *p < 0.05, **p < 0.01; n = 3. (F) A representative proliferation curve of mMSCs from three independent experiments. Data are expressed as the mean ± SD. *p < 0.05; n = 3. Abbreviations: Con., control; PN, PBN+NAC; CmMSCs, mMSCs on plastic as controls; EmMSCs, mMSCs on MEF-ECM; PCmMSCs, mMSCs on plastic in the presence of PBN and NAC; PEmMSCs, mMSCs on MEF-ECM in the presence of PBN and NAC.