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. 2011 Jul 5;4:127. doi: 10.1186/1756-3305-4-127

Figure 1.

Figure 1

Immunodetection of the putative fibrinogen-related proteins in tick haemolymph. 1A - Electrophoretically separated and electroblotted non-reduced D. marginatus haemolymph proteins immunostained using mouse anti-(DM)HA serum (lane 1) and rabbit anti-Dorin M serum (lane 2). Four proteins were detected with molecular weights of approximately 36, 79/80, and 290 kDa (marked with asterisks; the 79/80 kDa double-band is marked by one asterisk). 1B - Electrophoretically separated and electroblotted non-reduced R. appendiculatus (lane 1), R. pulchellus (lane 2), and R. sanguineus (lane 3) haemolymph proteins immunostained using mouse anti-(RA)HA serum. In each sample, three proteins were detected with size of 58, 75, and 185 kDa (asterisks). Furthermore, a protein band with molecular weight of 45 kDa was observed in R. pulchellus (arrow). 1C - Electrophoretically separated and electroblotted non-reduced R. appendiculatus (lane 1), R. pulchellus (lane 2), and R. sanguineus (lane 3) haemolymph proteins immunostained using rabbit anti-Dorin M serum. Proteins with molecular weight of 75 and 185 kDa were detected, as in the case of anti-(RA)HA serum (asterisks).