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View full-text article in PMC

. 2011 Jun 7;11:225. doi: 10.1186/1471-2407-11-225

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Copyright ©2011 Lee et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Reconstitution of human cord blood derived T lymphocytes in NOD/SCID mice. Cord blood mononuclear cells (1 × 10⁷cells) were injected into the peritoneum of newborn NOD/SCID mice. A, representative flow cytometry data is shown in indicated hematopoietic organs from NOD/SCID mice injected with cord blood mononuclear cells at 6 weeks after transplantation. B, cells from cord blood or spleen of cord blood injected NOD/SCID mice were compared the expression of CD38 and CD62L expression in CD3⁺cells. C, splenocytes from cord blood injected NOD/SCID mice were gated on CD3⁺splenocytes, and analyzed for TCRαβ expression. D, immunohistochemical analysis of the thymus (left) and spleen (right) was performed using antibodies to CD4 (FITC) and CD8 (PE). Hoechst dye was used for counterstaining of cell nuclei.