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. 2003 Dec 26;101(1):296–301. doi: 10.1073/pnas.2434651100

Fig. 3.

Fig. 3.

Anti-HMGB1 antibodies protect against lethality caused by cecal perforation. (A) BALB/c mice were randomly grouped (18-25 mice per group) and subjected to CLP. Mice received neutralizing IgG-purified (or, in some experiments, affinity-purified) anti-HMGB1 antibodies twice daily for 3 days at 60 or 600 μg per mouse (i.p.) beginning 24 h after surgery. Irrelevant nonimmune rabbit IgG was used as control. *, P < 0.03 vs. control, as tested by two-tailed Fisher's exact test. •, anti-HMGB1 antibody, 600 μg per mouse (24-h delay); ▴, anti-HMGB1 antibody, 60 μg per mouse (24-h delay); ○, IgG control. (B) Anti-HMGB1 antibody treatment (600 μg per mouse) was initiated at 12, 24, or 36 h after surgery, twice a day, and continued for 3 days. * and #, P < 0.05 vs. IgG control group. ▴, anti-HMGB1 antibody (12-h delay); •, anti-HMGB1 antibody (24-h delay); ★, anti-HMGB1 antibody (36-h delay); ○, IgG control.