Fig. 6.

A box protects against lethality caused by LPS or cecal perforation in mice. (A) BALB/c mice were randomly grouped (18 mice per group) and challenged with an LD₇₅ dose of LPS (15 mg/kg of body weight, i.p.). A box or GST control was administered at 600 μg per mouse (in 300 μl of sterile PBS) either immediately or 2 h after LPS injection. Two additional doses were given (i.p.) at 12 and 24 h after the first treatment. * and #, P < 0.05 vs. GST control group as determined by two-tailed Fisher's exact test. •, A box (0-h delay); ▴, A box (2-h delay); ○, GST control. (B) BALB/c mice (15-25 mice per group) were subjected to CLP. At 24 h after the operation, mice were given recombinant HMGB1 A box or GST control (60 or 600 μg per mouse per injection, i.p.) twice daily for 3 days, beginning 24 h after surgery. Animal survival was monitored for up to 2 weeks to ensure that no late death occurred. *, P < 0.03 vs. GST control as tested by two-tailed Fisher's exact test. ▴, A box 60 μg per mouse (24-h delay); •, A box 600 μg per mouse (24-h delay); ○, GST control.