Figure 5.
Soluble sugars in laser-microdissected tissues of B. napus measured by ion chromatography. (a) Typical chromatograms of sugar standard, LM-sample and blank. Melezitose was added during extraction to all samples as an internal standard. (b-d) Levels of glucose (b), fructose (c) and sucrose (d) in LM-samples of 1 element per hypocotyl, inner cotyledon and outer cotyledon (minimum tissue amount ~0.0063 mm3). Mean values +/- standard deviation are shown (n = 5). Stars in b-d indicate statistically significant differences versus hypocotyl according to t-test (p < 0.01).