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. 2003 Dec 18;101(1):331–336. doi: 10.1073/pnas.0303510101

Fig. 7.

Fig. 7.

(A) PMN exacerbation of KA-induced neuron death is blocked by protease inhibitors. PMNs were cocultured at 1/1 and 2/1 E/T ratios (± PIC) with KA-treated hippocampal cells for 24 h. Neuron viability was determined by ABTS assay. Addition of PIC caused a significant increase in neuronal survival (**, P < 0.001 vs. KA/PMN-1,2; n = 30-40 per bar). (B) Immunocytochemical analysis of PIC treatment and blocking of PMN exacerbation of neuron death. Cells were treated as described in A. Cultures were then immunostained for neurons (MAP-2) followed by a Wright stain to visualize PMN (arrows in B3). The addition of PIC successfully prevented PMN exacerbation of KA-induced neuron loss (B1, KA alone; B2 and B3, KA plus PMN-2/1 E/T ratio; B4, KA plus PMN plus PIC).