TABLE 4.
M3 receptor-mediated stimulation of [3H]inositolphosphate accumulation in HEK 293 cells expressing Gα15
The parameters were estimated from the agonist concentration-response curves shown in Fig. 7.
| Agonist | Log Kb | Log Kaa | Log Kobsb | Log Kb/Kac | Log Relative Efficacyd |
|---|---|---|---|---|---|
| Oxotremorine-M | 7.64 ± 0.27 | 2.68 | 4.00 ± 0.13 | 3.6–4.9 | 0.00 |
| Carbachol | 7.20 ± 0.27 | 3.31 | 3.74 ± 0.14 | 3.4–3.9 | −0.18 |
| McN-A-343 | 6.03 ± 0.21 | 5.25 | 5.25 ± 0.20 | 0.78 | −2.85 |
a
A minimum estimate of log Ka.
b
The Kobs values of carbachol and oxotremorine-M were estimated by the method of partial receptor inactivation as described in the text, and that of McN-A-343 by regression analysis of the data in Fig. 7 using eq. 37.
c
The range of values represents log Kb/Kobs − log Kb/Ka.
d
The log τ value of each agonist normalized relative to that of oxotremorine-M.