Fig. 2.

Assessment of constitutive activity shown by IP production by mutants of Lys¹²¹. COS-7 cells were transfected with 100 ng of WT or mutant cDNA as described under Materials and Methods. Mutants were incubated in media alone or rescued with pharmacoperone (In3, Q103); pharmacoperones In3 and Q103 were washed out, and IP production was measured in response to media alone (no agonist). The dashed horizontal line shows basal level of vector only. Means ± S.E.M.s are shown for three independent experiments, each performed in replicates of four.