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. 2011 Aug;338(2):430–442. doi: 10.1124/jpet.111.180869

Fig. 3.

Fig. 3.

Assessment of constitutive activity shown by IP production of mutants of Arg38 (A) and Asp98 (B). COS-7 cells were transfected with 100 ng of WT or mutant cDNA as described under Materials and Methods. Mutants were incubated in media alone or rescued with pharmacoperone In3 (A and B). In3 was then washed out, and IP production was measured in response to media alone (no agonist added). C, IP production in response to agonist (10−7 M buserelin, a saturating dose) with or without In3 rescue. COS-7 cells were transfected with 20 ng of WT or mutant cDNA plus 80 ng of empty vector. The dashed horizontal line shows the basal level of vector only. Means ± S.E.M.s are shown for three independent experiments, each performed in replicates of four.