Fig. 9.
IP production by WT GnRHR and various mutants expressed in COS-7 cells in response to 10−9 M agonist (buserelin) and in the presence of Q89. A, human: COS-7 cells were transiently transfected with 10 ng of human WT or human mutant cDNA plus 90 ng of empty vector, and IP production was determined as described under Materials and Methods. B, rat: COS-7 cells were transiently transfected with 5 ng of rat WT or rat mutant cDNA plus 95 ng of empty vector, and IP production was determined as described under Materials and Methods. Means ± S.E.M.s are shown for three independent experiments, each performed in replicates of four.