Fig. 2.

Tissue-specific expression patterns of miRNAs cloned from rat cortex. Northern blots of total RNA isolated from adult rat tissues were assayed with probes against 12 miRNAs. Eight miRNAs were expressed predominantly in the cortex and cerebellum. Two miRNAs are shown in a: mir-129-2^* and mir-124a, previously cloned from mouse (14); the others (not shown) with brain-restricted expression patterns include the rat homologue of mir-103 cloned from the Gemin3-Gemin4 - eIF2C2 complex in human HeLa cells (16), mir-128 from mouse (14), and four previously uncharacterized miRNAs (mir-323,-326, -329, and -344). Four miRNAs exhibit a broader expression pattern. Two miRNAs are shown in b: mir-191 and mir-324-5p; the others with broad expression patterns were mir-335 and mir-151^*. The tissues analyzed were cortex (cx), cerebellum (cb), kidney (kd), heart (ht), liver (lv), thymus (th), lung (lu), ovary (ov), and testis (ts).