Fig. 4.

N-MeVal-4-CsA inhibits in vivo angiogenesis in Matrigel plugs. A, mice with subcutaneous Matrigel plugs containing recombinant murine bFGF and VEGF₁₆₄ were treated with either vehicle, N-MeVal-4-CsA (25 mg/kg), or CsA (25 mg/kg). The average number of erythrocyte-filled vessels per 200× field within one field depth of the plug surface around the entire perimeter was determined by a blinded observer and plotted. Each data point represents a single mouse. Bars represent the group mean (n = 7 for vehicle; n = 8 for N-MeVal-4-CsA and CsA). P values are shown. B, representative microscopic images of MAS trichrome-stained Matrigel plugs with the surface positioned in the upper left-hand corner. Arrowheads indicate erythrocyte-filled vessels. Scale bars, 100 μm.