Fig. 1.

α₁-AR modulation of IL-1β production from LPS-challenged primary human monocytes. A, representative immunoblot of resolved total cell lysates from isolated primary human monocytes. The 42-kDa actin band is shown as a loading control. B, quantitative analysis of all immunoblots (n = 7) showed increased expression of IL-1β from cells treated only with LPS (8.9 ± 3.1-fold). In addition, there was a synergistic increase in IL-1β generation from cells treated with PE plus LPS (31.0 ± 5.8-fold). There were no differences in IL-1β production from cells treated with PE (1.0 ± 0.6-fold) alone. ★, p < 0.05 versus control; #, p < 0.05 versus LPS.