Fig. 3.

Temporal p38 MAPK responses in monocytes/macrophages initiated by concurrent α₁-AR and TLR4 stimulation. A, representative immunoblot of resolved THP-1 total cell lysates incubated concurrently with PE and LPS. B, quantitative analysis of all immunoblots (n = 3) showed increased phosphorylated p38 MAPK from monocytes treated for 30 min (4.2 ± 0.6-fold), 60 min (4.6 ± 1.3-fold), and 120 min (8.2 ± 0.6-fold). There were no differences in activated p38 MAPK generated from monocytes treated for 15 min (2.4 ± 0.3-fold) and 180 min (2.1 ± 0.4-fold). C, representative immunoblot (n = 3) of resolved THP-1 total cell lysates incubated with LPS alone. D, representative immunoblot of resolved PMA-differentiated THP-1 total cell lysates incubated concurrently with PE and LPS. E, quantitative analysis of all immunoblots (n = 3) showed increased phosphorylated p38 MAPK from macrophages treated for 15 min (7.7 ± 1.2-fold) and 30 min (10.7 ± 1.6-fold). There were no differences in activated p38 MAPK generated from macrophage cells treated for 5 min (1.5 ± 0.6-fold), 60 min (3.4 ± 1.0-fold), and 120 min (3.2 ± 0.2-fold). F, representative immunoblot (n = 3) of resolved PMA-differentiated THP-1 total cell lysates incubated with LPS alone. In all immunoblots, the 43-kDa nonphosphorylated p38 MAPK band is shown as a loading control. ★, p < 0.05 versus control.