Fig. 6.

p38 MAPK activation is necessary for the α₁-AR synergistic modulation of IL-1β production. A, representative immunoblot of resolved THP-1 total cell lysates overexpressing the empty vector or DN p38 MAPK plasmid constructs. The 42-kDa actin band is shown as a loading control. B, quantitative analysis of all immunoblots (n = 3) normalized to basal levels of cells transfected with empty vector showed increases in IL-1β generated from similarly transfected cells treated with PE plus LPS (7.2 ± 2.5-fold). There were no IL-1β differences in DN p38 MAPK-transfected cells observed from control (0.8 ± 0.4-fold) or PE plus LPS treatments (1.1 ± 0.2-fold). ★, p < 0.05 versus empty vector control.