TABLE 3.

Hepatic PAI-1 mRNA expression after APAP overdose in HIF-1α^+fl/+fl and HIF-1α^Δ/Δ mice

HIF-1α^+fl/+fl and HIF-1α^Δ/Δ mice were treated with SAL or APAP then killed after 2, 6, or 24 h. Real-time PCR was used to analyze liver tissue for the expression of PAI-1 mRNA. For each sample, the copy number of PAI-1 was normalized to that of HPRT. Data represent mean of the PAI-1/HPRT ratio ± S.E.M. of n = 3–6.

Mouse	Real-Time PCR (PAI-1/HPRT ratio) ×100
	SAL			APAP
	2 h	6 h	24 h	2 h	6 h	24 h
HIF-1α+fl/+fl	1 ± 0.5	1.0 ± 0.5	0.03 ± 0.1	16 ± 7a	14 ± 7a	12 ± 4a
HIF-1αΔ/Δ	0.1 ± 0.03b	0.2 ± 0.03b	0.6 ± 0.2	26 ± 20a	2.9 ± 1b	11 ± 5a

^a Significantly different from SAL-treated mice of the same genotype.

^b Significantly different from corresponding HIF-1α^+fl/+fl mice.