Fig. 1.

Cell surface expression of mGlu₅, Δ5, and Δ5Δ. (A) Schematic representation of mGlu₅, Δ5, and Δ5Δ and location of the sites of truncation. The open box represents the HA tag, and the gray box corresponds to the signal peptide of mGlu₅.(B) Surface expression of mGlu₅, Δ5, and Δ5Δ in HEK 293 cells was detected by immunofluorescence on nonpermeabilized cells. (C) Quantification of cell surface expression of mGlu₅, Δ5, and Δ5Δ by ELISA on intact cells. Cells were transfected with 0.6, 5, and 5 μg of plasmids expressing mGlu₅, Δ5, and Δ5Δ, respectively.