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Wo interacts with SlCycB2 in vivo. (A) Interaction between Wo and SlCycB2 in BiFC assays. YFP fluorescence was detected only when pUCSPYNE-Wo was coexpressed with pUCSPYCE-SlCycB2. (B) Interaction of Wo and SlCycB2 in transformed AH109 cells grown on SD/-Leu/-Trp (a) and SD/-Ade/-His/-Leu/-Trp (b). 1, pGBKT7-53+pGADT7-RecT (positive control); 2, pGBKT7-Lam+pGADT7-RecT (negative control); 3, pGBKT7-Wo+pGADT7-SlCycB2.
