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Prep1i/i MEF response to radiation-induced DNA damage. (A) Time course by immunofluorescence of γH2AX accumulation after γ-irradiation with 3Gy. At least 50 cells per slide were analyzed. *P < 0.01. (B) Immunoblotting analysis of extracts from WT and Prep1i/i MEFs at various times after 3Gy γ-irradiation, with specific anti–γ-H2AX, phospho-Atm, Atm, Prep1, and vinculin antibodies.
