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. 2011 Jun 22;108(29):11936–11941. doi: 10.1073/pnas.1105362108

Fig. 2.

Fig. 2.

The 3′-UTR of Pax3 is regulated by miR-1 and miR-206. (A) Alignment of target sites TS1 and TS2 with miR-1 and miR-206; Materials and Methods provides the genomic positions. (B) Normalized luciferase activity shown for Pax3–3′-UTR sensor constructs cotransfected with miR-1, miR-206, or miR-140 as indicated by (+). Activity was plotted relative to control (no miRNA). Experiments were repeated four times with triplicate samples and two independent DNA plasmid preparations. Error bars represent SE (n = 12). The constructs used are indicated above the graphs; TSm represents constructs with mutant target sites. (C) qPCR of Pax3 expression in RuGli cells. Pax3 transcript levels in mock-transfected control cells (lane 1) were reduced after transfection of miR-206 (lane 3) and miR-1 (lane 4). Cotransfection of antimiR-206 (AM) with miR-206 (lane 2) restored Pax3 expression. (D) Western blot analysis showing reduced Pax3 protein in RuGli cells transfected with miR-206 (lane 3) and miR-1 (lane 4). Protein levels were unaffected after cotransfection of antimiR-206 (AM) with miR-206 (lane 2).