Skip to main content
. 2003 Dec 22;101(1):396–401. doi: 10.1073/pnas.0303329101

Fig. 1.

Fig. 1.

Tyr-253 is not required for the hypotonicity-induced activation of TRPV4. (A) Effect of stimulation with 25% hypotonic solution on [Ca2+]i in nontransfected (NT) HEK cells (dashed line) and cells transfected with the Y253F mutant (solid line). (B, left) Average [Ca2+]i increases induced by hypotonic stimulation in NT cells (n = 31), WT TRPV4-transfected cells (HTS, n = 32), and TRPV4-transfected cells treated with the tyrosine kinase inhibitors PP1 (10 μM; n = 22) or genistein (Gen; 100 μM; n = 23). (B, right) Average [Ca2+]i increases in Y253F-transfected cells induced by hypotonic cell swelling (HTS; n = 22), 1 μM4α-PDD (4αPDD; n = 18), 10 μMAA(n = 21), and heating to 42°C (Heat; n = 22). For every condition, data from at least three independent experiments were pooled.