Table 1. β-Xanthophyll production and β-ring hydroxylation in leaf tissue of wild-type and carotenoid hydroxylase mutants.
Genotype | β-Xanthophylls* | Hydroxylated β-rings† |
---|---|---|
Ws | 54.0 ± 2.7a‡ | 48.5 ± 1.0a |
Col | 60.7 ± 7.6a | 48.7 ± 0.9a |
b1 b2 | 20.5 ± 4.8b | 40.2 ± 1.4b |
lut1—2 b1 b2 | 26.5 ± 3.4b | 33.6 ± 2.4c |
lut1—3 b1 b2 | 28.3 ± 4.6b | 31.1 ± 1.2c |
Total carotenoids were extracted from 5-week-old plants and quantified by HPLC as described (12).
β-Xanthophylls are the sum of zeaxanthin, antheraxanthin, violaxanthin, and neoxanthin as mmol of pigment/mol of chlorophyll a + b
Data are given as percentage of total ring hydroxylation
All values are means ± SD (n = 6). Values marked with the same letters are not significantly different from each other within a column (Student's t test, P > 0.05)