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. 2011 Jun 28;108(29):11854-11859. doi: 10.1073/pnas.1107844108

Fig. 2.

Fig. 2.

Three newly identified dual-Cys biliprotein photosensors from Nostoc punctiforme. (A) Absorbance spectra are shown for VO1 with PCB. (B) Photochemical difference spectra are shown for VO1 with PCB (blue) and PΦB (red) chromophores. (C) Absorbance spectra are shown for UB1 with PCB. The photoequilibrium spectrum produced by 400 nm illumination is dashed orange, whereas that produced by 334 nm illumination is solid orange. (D) Photochemical difference spectra are shown for UB1 with PCB (blue) and PΦB (red) chromophores. (E) Absorbance spectra are shown for TP1 in the dark-adapted ground state (purple), after illumination with 400 ± 35 nm light (orange), and after subsequent incubation in darkness at 25 °C for 20 min (red). (F) Difference spectra are shown for photoconversion of TP1 with 400 nm light (blue) and for the subsequent thermal evolution of the 15E photoproduct (deep purple).