Fig. 2.
E-cadherin expression regulates cell density-dependent redistribution of YAP from nucleus to cytoplasm. (A and B) Parental MDA-MB-231 cells and stable MDA-MB-231 clones, which contain doxycycline-inducible full-length E-cadherin, E-cadherin Δβ-catenin, E-cadherin–α-catenin fusion, or E-cadherin Δp120 transgene were seeded on fibronectin-coated coverslips in 24-well plates sparsely (1 × 104 cells) or densely (2 × 105 cells). Wild- or mutant-type E-cadherin expression was induced by the treatment of 2 μg/mL of doxycycline for 2 d. Cells were cultured under sparse (A) or dense (B) conditions, and endogenous YAP was stained by anti-YAP antibody. (Magnification, 200×.) (C) Subcellular localization of YAP in A and B was quantified using Blobfinder.