Fig. 3.
Measured diffusion coefficients D for BSA solutions in D2O at T = 280 K (blue circles on upper curve) and translational diffusion coefficients Dt (purple circles on lower curve) computed from D using Eq. 4 and the theoretical rotational diffusion coefficient from ref. 19. The lines are polynomial fits. The dilute limit Dt(0) (diamond symbol) is calculated from results of dynamic light scattering (41). The noncoincidence of Dt(0) and the fit to D indicates a significant rotational contribution. After separation of the rotational contribution, the translational diffusion coefficient Dt is in accordance with the dilute limit, supporting the validity of our approach.