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Direct relationship between free testosterone and free estradiol. ●, Calculated free testosterone and free estradiol (r = 0.24, P = 0.001). ○, Free testosterone and free estradiol directly measured by equilibrium dialysis (r = 0.24, P = 0.02). Upper limit of normal for calculated free estradiol is 0.13 ng/dL and for measured free estradiol is 0.045 ng/dL.
