FIG. 4.

Macrophage depletion by liposome clodronate had no effect on HFD-induced adipocyte cell death. Wild-type mice fed an NCD (WTNCD) were then fed an HFD and concomitantly injected weekly with liposome PBS or liposome clodronate for 8 weeks (A, C, E, G). Mice fed an HFD for 8 weeks were injected weekly with liposome PBS or liposome clodronate for an additional 7 weeks (B, D, F, H). These mice were continually maintained on the HFD. A and B: Representative FACS analyses of the stromal vascular fraction (SVF) isolated from epididymal adipose tissue from a total of four to seven independent determinations. C and D: Adipose tissue from three independent mice was extracted and immunoblotted for perilipin, ATGL, and actin. E and F: A representative TUNEL staining section shows positive nuclei (arrows). G and H: Quantification of TUNEL-positive cells are mean ± standard error of the mean for four to seven mice per group with 200–500 nuclei per counted per individual mouse section. N.S. as determined by Student t test. (A high-quality digital representation of this figure is available in the online issue.)