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Cumulative distribution of islets from three rats after simultaneous islet oxygenation (pimonidazole immunostaining) and blood flow measurements (microspheres)
| Pimonidazole immunostaining |
||
|---|---|---|
| Negative | Positive | |
| Islets containing microspheres | ||
| No | 1,292 | 378 |
| Yes | 48 | 2 |
The pimonidazole immunostaining was dependent on the simultaneous blood flow measurements (χ2 test, P < 0.05), suggesting that pimonidazole preferentially accumulates in islets with low blood perfusion.
